Естественная рекомбинация у пикорнавирусов

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Биологические науки
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1. Естественная рекомбинация широко распространена у родов Hepatovirus (вирус гепатита А) и Kobuvirus (вирус Айчи) семейства Picornaviridae.

2. Рекомбинация у родов Hepatovirus (вирус гепатита А) и Kobuvirus (вирус Айчи) выявляется во всех участках генома с приблизительно одинаковой частотой, в отличие от других пикорнавирусов.

3. На основании молекулярно-эпидемиологических данных показана репродуктивная изоляция генотипов вируса гепатита А. Каждый генотип вируса гепатита, А удовлетворяет репродуктивному критерию в биологической концепции вида и может представлять самостоятельный таксономический вид.

4. Закономерности рекомбинации у парэховирусов в целом сходны с таковыми у энтеровирусов и большинства других пикорнавирусов. В пределах области генома, кодирующей поверхностные белки капсида, рекомбинация обнаруживается редко, а в кодирующей неструктурные белки часто, чаще всего на границах структурной области генома в областях, кодирующих белки VP0 и 2А.

5. Впервые зафиксирована рекомбинация внутри области генома, кодирующей поверхностные белки капсида парэховирусов, между вирусами, принадлежащими к одному серотипу.

6. Впервые для вирусов с несегментированным геномом показана географическая специфичность варианта фрагмента генома независимо от других участков генома, возможная благодаря частой рекомбинации. Монофилетический вариант фрагмента генома парэховирусов, кодирующий протеазу ЗС, преобладал на протяжении порядка 150 лет у вирусов четырех различных серотипов, циркулировавших на территории Бразилии.

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Содержание

Список сокращений.

Цель работы.

Задачи работы:.

Научная новизна.

Практическая значимость работы.

Обзор литературы.

Строение генома пикорнавирусов.

Трансляция вирусного генома.

Репликация генома.

Сборка вириона.

Классификация пикорнавирусов.

Эволюция пикорнавирусов.

Рекомбинация.

Естественная рекомбинация.

Роль рекомбинации в эволюции.

Материалы и методы.

Выравнивание нуклеотидных последовательностей.

Реконструкция филогении методом Neighbor-joining.

Бутстрэп анализ.

Матрицы филогенетической совместимости.

Анализ филогенетического группирования.

Графики сходства нуклеотидных последовательностей и бутскан-анализ.

Консенсусное филогенетическое дерево.

Реконструкция филогении с использованием Байесового вывода вероятности.

Результаты.

Матрицы филогенетической совместимости.

Бутскан-анализ и графики сходства последовательностей.

Филогенетические взаимоотношения в разных областях генома между представителями вируса гепатита А.

Филогенетические взаимоотношения для расширенной выборки вируса гепатита, А в двух участках генома.

Анализ филогенетического группирования парэховирусов человека в различных участках генома.

Филогенетические взаимоотношения между парэховирусами человека в структурной области генома.

Филогенетические взаимоотношения между парэховирусами человека в неструктурной области генома.

Обсуждение результатов.

Рекомбинация у вируса гепатита А.

Репродуктивная изоляция генотипов вируса гепатита А.

Классификация вируса гепатита, А и рекомбинация.

Распределение точек рекомбинации у вируса гепатита, А и других пикорнавирусов.

Рекомбинация у парэховирусов человека.

Эндемичность ЗС-протеазы парэховирусов на территории Бразилии.

Рекомбинация и молекулярное типирование пикорнавирусов.

Рекомбинация и видовая идентичность пикорнавирусов.

Выводы.

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